Synthetic oligodeoxynucleotide purification by polymerization of failure sequences.
نویسندگان
چکیده
Synthetic oligodeoxynucleotide is purified by capping failure sequences with an acrylated phosphoramidite followed by polymerization and product extraction. The method is suitable for large scale oligonucleotide drug purification.
منابع مشابه
A Highly Convenient Procedure for Oligodeoxynucleotide Purification
Purification of synthetic oligodeoxynucleotides (ODNs) is simply achieved by capping failure sequences with a polymerizable phosphoramidite followed by polymerization. In this article, the reduction of the amount of polymerization monomer to drastically increase ODN extraction efficiency, the use of a centrifugal filterunit to ease the extraction process and the notification of using fresh phos...
متن کاملDissolving Pulp (alpha-cellulose) from Corn Stalk by Kraft Process
Corn stalk can be used as an alternative non-wood raw material to produce cellulosic dissolving grade pulp. In this work, the potential of corn stalk in the production of dissolving pulp (alpha-cellulose) was investigated. It was collect ed from a corn stalk farm of Kermanshah, Iran. The sequences of experimental work were: water and acid prehydrolysis at 160˚C for 30min, followed by Kraft pulp...
متن کاملاستخراج و بهینه‌سازی تخلیص چند مرحله‌ای آنزیم تبدیل کننده آنژیوتانسین I(ACE) از ریه خرگوش
Angiotensin-converting enzyme(ACE)(EC: 3.4.15.1) is a peptidyl dipeptide hydrolase that removes the carboxyl terminal (His-Leu) from angiotensin I to produce the octapeptide angiotensin II. Due to the importance of ACE and its active site-directed inhibitors in the pathogenesis and treatment of cardiovascular disorders such as hypertension and heart failure, ACE purification and ass...
متن کاملUse of oligodeoxynucleotide probes for quantitative in situ hybridization to actin mRNA.
We have employed an analytical approach for the development of an in situ hybridization methodology using synthetic oligodeoxynucleotide probes for actin messenger RNA detection in cultures of chicken fibroblasts and myoblasts. The methodology developed shows that oligonucleotides can complement the use of nick-translated probes in specific situations. Since they can be made to specific nucleic...
متن کاملSorption Study of Acid Blue in Aqueous Solution by Synthetic Poly Urea-Formaldehyde
Poly urea-formaldehyde was synthesized simply and rapidly from urea and formaldehyde by condensation polymerization and studied for the sorption of trace acid blue from aqueous solution. The optimum pH value for sorption of the acid blue was 4. Experimental results showed that the sorption capacity of poly urea-formaldehyde for acid blue was determined. The synthetic polymer can be reused for 5...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical communications
دوره 47 4 شماره
صفحات -
تاریخ انتشار 2011